Assoc. Prof. Dipl.-Ing. Josef Velíšek, Ph.D. head of laboratory, academic staff velisek@frov.jcu.cz +420 38777 4625     +420 725 787 931	curriculum vitae CS curriculum vitae EN publication			Dipl. Biol. Christoph Steinbach, Ph.D. research scientist steinbach@frov.jcu.cz +420 38777 4622	curriculum vitae CS curriculum vitae EN publication
	Assoc. Prof. Dipl.-Ing. Hana Kocour Kroupová, Ph.D. head deputy, academic staff kroupova@frov.jcu.cz +420 38777 4621	curriculum vitae CS curriculum vitae EN publication			Dipl.-Ing. Pavel Šauer, Ph.D. research scientist psauer@frov.jcu.cz +420 38777 4622	curriculum vitae CS curriculum vitae EN publication
	Prof. Zdeňka Svobodová, MVD., DSc. research scientist zsvobod@frov.jcu.cz +420 38777 4625	curriculum vitae CS curriculum vitae EN publication			MSc. Latifeh Chupani, Ph.D. research scientist lchupani@frov.jcu.cz +420 38777 4715	curriculum vitae EN publication
	Dipl.-Ing. Olga Valentová academic staff valentova@frov.jcu.cz +420 38777 4754	curriculum vitae CS curriculum vitae EN publication			Dipl.-Ing. Josef Příborský external PhD student priborsky@frov.jcu.cz +420 38777 4763	curriculum vitae CS curriculum vitae EN publication
	Eliška Zusková, MVD., Ph.D. academic staff zuskova@frov.jcu.cz +420 38777 4621	curriculum vitae CS curriculum vitae EN publication			Ilona Prokopová technician prokopova@frov.jcu.cz +420 38777 4754
	Veronika Piačková, MVD., Ph.D. academic staff piackova@frov.jcu.cz +420 38777 4621; +420 734 269 018	curriculum vitae CS curriculum vitae EN publication			Dipl.-Ing. Marie Šandová technician msandova@frov.jcu.cz +420 38777 4625	curriculum vitae CS curriculum vitae EN
	Dipl.-Ing. Alžběta Stará, Ph.D. academic staff staraa01@frov.jcu.cz +420 38777 4622	curriculum vitae CS curriculum vitae EN publication			Dipl.-Ing. Michal Pech PhD student pechm@frov.jcu.cz
	Ing. Klára Zálohová professional staff kzalohova@frov.jcu.cz +420 38777 4754				Dipl.-Ing. Jan Chábera external PhD student chabej00@frov.jcu.cz
	MSc. Ali Asghar Baloch PhD student abaloch@frov.jcu.cz	curriculum vitae CS curriculum vitae EN publication			Dipl-Ing. Nikola Třešňáková PhD student tresnakova@frov.jcu.cz	curriculum vitae CS curriculum vitae EN publication
	Mgr. Pavlína Hucková laboratory assistant phuckova@frov.jcu.cz +420 38777 4622				Dr. Ehdaa Eltayeb Eltigani Abdelsalam, BVM, Ph.D. research scientist abdelsalam@frov.jcu.cz	curriculum vitae CS curriculum vitae EN publication
	Dipl.-Ing. Alžběta Strouhová PhD Student astrouhova@frov.jcu.cz

Laboratory unites experts in the field of Veterinary Medicine, Aquatic Toxicology, Hydrochemistry and Biochemistry, who are engaged in basic and applied research, university teaching, and consulting and advisory activities. Laboratory deals with the research programs of centre CENAKVA: WP 3 Innovation of intensive production methods of economically important fish species and species important for angling and WP 5 Innovation in monitoring the occurrence of extraneous substances in environment, evaluation of the influence on exposed organisms and possibilities of elimination in clearing processes.

The wokplace toxicology and hydrochemistry are important parts of this laboratory.

On workplace toxicology, acute toxicity test on fish, daphnia, freshwater green algae, and white mustard seeds are routinely performed. On the basis of obtained data, ecotoxicological properties of substances, chemical products and wastes are assessed. Moreover, for scientific purposes are performed sub-chronic and chronic toxicity tests on fish, crayfish and their early-life stages.
 

 

Workplace hydrochemistry performs analysis of basic parameters of water quality for research institutes and fish farmers. Every year laboratory successfully participates in inter-laboratory comparison tests organized by the Centre for Assessment of Laboratories ASLAB at T. G. Masaryk Water Research Institute, public research institution (TGM WRI) in Prague and obtain a certificate of participation. In this way, continual external control of both laboratories is guaranteed.
Staff of the laboratory also provides manufacturing and service of Combi sets which are used for field analysis of water. Moreover carries out analysis of total mercury in biological materials and sediments.

 

  

Prevention and therapy of fish diseases
This is mainly focused on verification and the development of strategies in the treatment of the most common diseases in aquaculture. Therapeutical and rearing procedures aimed at minimizing of losses caused by various pathogens are designed for fish farmers. At present attention is mainly paid to the possibility of using peracetic acid in the aquaculture, including verification of spectrum efficiency and application possibilities. Results of laboratory and field experiments were used for preparation of methodology entitled „Possibilities of using peracetic acid in the aquaculture ˮ.
The koi herpes virus (KHV) is an important issue that laboratory staff is engaged in. In cooperation with the Genetic Fisheries Centre of the faculty (FFPW) and Veterinary Research Institute (VRI Brno), testing of resistance of pure breeds and hybrids of common carp to KHV under laboratory conditions are performed. The aim is to identify breeds and hybrids, which have showed the highest survival in experimental infections, to expand their use in the production of fisheries and thus contribute to reduction of the risk of occurring and spreading of koi herpes virus in the Czech pond aquaculture.
Moreover, staff of the laboratory performs basic pathological and parasitological examinations of freshwater and aquarium fish for fish farmers.

  

Indicator of water quality	The amount of indicator standard
pH	6 – 8
O2 (mg.l-1)	min. 6
Biological oxygen demand BOD (mg.l-1)	6
Chemical oxygen demand CODCr (mg.l-1)	35
Total phosphorus Ptotal (mg.l-1)	0,20
Total nitrogen Ntotal (mg.l-1)	8
Ammonium nitrogen N-NH4 (mg.l-1)	0,5
Nitrite nitrogen N-NO2 (mg.l-1)	0,14
Nitrate nitrogen N-NO3 (mg.l-1)	7
Suspended solids SS (mg.l-1)	30

Results of five years monitoring showed that very problematic appears respect BOD value, that has been exceeded in more than 50% even in cases of very low initial stocking without feeding. In contrast, as the no problem appeared concentrations of total nitrogen and his forms. Water quality parameters such as CODCr, total phosphorus and suspended solids are negatively affected by the level of stocking and feed applications. The proportion of unsatisfactory values of different water quality parameters in relation to the initial stocking of fish are shown in the following graphs:

 

The staff of laboratory in collaboration with colleagues from the Veterinary and Pharmaceutical University Brno published a book Aquatic Toxicology for Fishermen in 2014. This book summarizes the knowledge about pollutants in aquatic environment and their effect on aquatic organisms, toxicity tests on aquatic organisms; investigation fish kill poisons and natural toxin.
 

Book Velíšek, J., Svobodová, Z., Blahová, et al., 2018. Aquatic Toxicology for Fishermen. Second updated edition, FROV JU, Vodňany, 658 s, ISBN 978-80-7514-069-2.

Staff of the laboratory cooperates also with Police of the Czech Republic, fishery associations and municipal authorities on the investigation of the causes of acute fish kills. Moreover, the laboratory monitors effects of pesticides on non-target aquatic organisms and records the acute fish kills in the Czech Republic in cooperation with the Ministry of Agriculture.