Ph.D. students of the FFPW USB will have a seminar at the IEEAIC Vodňany (Na Valše 207) on Monday 9^th March 2015 from 12,00 a.m. to 3:30 p.m.

On the program will be:

Dipl.- Ing. Pavel Šauer - Detection of multiple hormonal activities in aquatic environment using in vitro bioassays
Dipl.- Ing. Martina Milatová - Psychoactive compounds in aquatic environment and their effects on fish
M.Sc. Pham Thai Giang - Biological effects of anthropogenic pollutants present in recipients of treated sewage water
M.Sc. Azadeh Mohagheghi Samarin - Oxidative Stress and Fish Egg Quality
M.Sc. Mariia Uzhytchak - Morphology, ecology and other biological characteristics of endemic European crayfish Astacus pachypus (Rathke, 1837)
Dipl.- Ing. Josef Příborský - Effect of anaesthetics on fish
Dipl.- Ing. Petr Svačina - Optimization of the culture in burbot (Lota lota) under controlled conditions

and

one other seminar:

Dipl.-Ing. Lukáš Valihrach, Ph.D. – z IBT AS CR v.v.i. - Single-Cell Gene Expression Analysis

The reverse transcription real-time polymerase chain reaction (RT-qPCR) has become the method of choice for the quantification of mRNA. Traditionally, when studying expression, a large number of cells in one sample is analyzed. Such approach quantifies just average response of cells. However, biological tissues are heterogeneous, being composed of different cell types. Therefore, a new technique for single cell expression profiling was developed. During the seminar, the workflow for single cell RT-qPCR analysis will be presented and discussed.

You are cordially invited.